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OBJECTIVE@#To study the association of microRNA expression before and after drug therapy with clinical symptoms in children with attention deficit hyperactivity disorder (ADHD).@*METHODS@#A total of 80 previously untreated children with ADHD who were diagnosed from May 2017 to October 2018 were enrolled. The children who were willing to receive drug therapy were randomly divided into concerta-treated group with 31 children and strattera-treated group with 33 children. The children who were unwilling to receive treatment were enrolled as the untreated group with 16 children. A total of 60 children who underwent physical examination during the same period of time were enrolled as the healthy control group. SNAP-V score was determined at initial diagnosis and 3 and 6 months of follow-up. Serum samples were collected from the children with ADHD and the healthy control group. Quantitative real-time PCR was used to measure the relative expression of miR-4566-3p and miR-7641.@*RESULTS@#The repeated measures analysis of variance showed that the SNAP-V score of attention deficit symptoms were different among the two treatment groups and the untreated group at the first visit and 3 months and 6 months after treatment (P0.05), and there was no interaction between the time factor and the grouping factor (P>0.05). The SNAP-V score of attention deficit symptoms was negatively correlated with the relative expression of miRNA-4655-3p and miRNA-7641 (r=-0.314, -0.495 respectively; P<0.05) in ADHD children after drug treatment.@*CONCLUSIONS@#Drug therapy can significantly improve the clinical symptoms of children with ADHD. The expression of miR-4655-3p and miR-7641 in serum can be used as biomarkers for the diagnosis and outcome evaluation of ADHD.
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Objective: To explore the differences of physicochemical properties, antibacterial effect and clinically curative effect between nano silver combined activated carbon gel dressings and traditional dressings in change dressing at puncture site of ascites drainage. Methods: 94 patients with seroperitoneum who were implemented abdominocentesis were divided into observation group (50 cases, who were applied NSAC dressing) and control group (44 cases, who were traditional dressing) as random number table. The inhibition zone method was adopted to test the actual bacteriostasis of two dressings, and the permeability, isolation function of protective screen and adhesive force of the two dressings were compared, respectively. Besides, the maximum use time, infection rate, errhysis degree, changed dressing times in 1 week and the loose situation in changing dressing at puncture site of drainage about the two dressings were compared. Results: The permeability, adhesive force, anti-invading for outside world of dressing in observation group were 3.8 times, 2 times and 1.7 times of that of control group, respectively. At the aspect of inhibition zone, the result of observation was 1.82 times of that of control group. And the maximum usage time of observation group was (8.4±1.8)d, and it was significantly higher than that of control group (5.3±1.2)d, (t=9.683, P<0.05). The difference of times of changed dressing between observation group (1.2±0.3 times) and control group (2.7±0.8 times) was statistically significant (t=-12.318, P<0.05). The loose rate, infection rate and errhysis rate of III grade of observation group (11.2%,3.33% and 2.0%) were significantly better than that of control group (29.5%, 18.2% and 15.9%) (x2=4.468, x2=4.951, x2=6.616, P<0.05). Conclusion:Compared to traditional dressing, novel nano silver conbined activated carbon gel dressings has obvious superiority in physicochemical properties, antibacterial effect and clinically curative effect, and it is worth to be extensively popularized in changing dressing for ascites drainage at puncture site.
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Objective To evaluate vitamin D nutritional status and its relationship with ultrasonic bone mineral density among children in Wenzhou City.Methods Overnight fasting venous blood samples of 546 children were collected to measure the concentration of serum 25 -hydroxyvitamin D (25 (OH)D),and bone mineral density was measured.Results The average concentration of serum 25 (OH)D was 25. 42 ±0. 590 ng/mL,25. 09% of participants were found with sufficient vitamin D,the rate of severe deficiency,deficiency and insufficiency were 1. 47%,32. 60% and 40. 84%respectively.The concentration of 25 (OH)D in different age groups was statistically significantly different (P<0. 01 ). Vitamin D nutritional status of the older groups was poor than that of the younger ones.In the group of children aged 7 to 1 1 and 11 to 14,the levels of 25(OH)D of boy was significantly higher than that of girl.Compared to those with sufficient vitamin D,children with vitamin D deficiency or insufficiency had lower bone mineral density(BMD)(P<0. 05 ).The level of 25(OH)D was correlated with BMD (r=0. 197,P<0. 01).After adjusted age and BMI,there was still a positive correlation between 25(OH)D level and BMD (r=0. 115,P<0. 01).Conclusions The vitamin D level of children in Wenzhou area is low.The level of serum 25 (OH)D may be related to BMD .More attention should be paid to the supplementation of vitamin D.
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<p><b>OBJECTIVE</b>To investigate the effect of adenovirus-mediated endostatin gene transfer on transplanted lung cancer in mice and its mechanism of action.</p><p><b>METHODS</b>Transplant tumor model was induced by subcutaneous inoculation of 2 x 10(6) Lewis lung cancer (LLC) cells into the back of C57BL/6 mice. The mice were treated by intratumoral injection of 2 x 10(9) pfu Ad-mEndostatin. The expression of endostatin in situ and its maintaining time were detected by immunohistochemistry and Western Blot, respectively. The endostatin level in serum was determined by ELISA . The inhibition of tumor growth and changes of survival were recorded and the microvessel density (MVD) was determined by histochemical stainingwith CD31 and CD105 antibodies. The tumor apoptosis was observed by electron microscopy.</p><p><b>RESULTS</b>In comparison with controls, intratumoral injection of Ad-mEndostatin significantly inhibited the tumor growth and metastasis, and prolonged the survival rate of mice (P < 0.05). Strong positive expression of mEndostatin was seen in the tumor tissue after injection of Ad-mEndostatin, immunhistochemically ostained by mouse endostatin monoclonal antibody, while the control groups showed only very low expression or absence. Serum endostatin concentration was 1540 +/- 560 ng/ml at the second week of administration, the expression of endostatin diminished a month later. The microvessel density (MVD)) decreased from 42.4 +/- 4.8 to 10.5 +/- 3.2 per x 200 magnificetion microscopic field by CD10 staining and from 68.5 +/- 4.5 to 37.5 +/- 4.6 by CD31 staining, respectively (P < 0.05). More apoptotic tumor cells were seen under the transmission electron microscope.</p><p><b>CONCLUSION</b>Endostatin gene therapy mediated by adenoviral vector efficiently induces expression of endostatin in vivo, and inhibits the growth and metastasis of tumor. It is concluded that its action is targeted to tumor neovasculature and the mechanism is inhibition of tumor angiogenesis.</p>